HeLaS3 also is adherent, but it can be in suspension culture. Cells can grow denser.
HeLa S3 is a clonal derivative of the parent HeLa line (see ATCC CCL-2). S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura. [22814].
The HeLa S3 clone has been very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition, and plaque-forming ability.The cells are positive for keratin by immunoperoxidase staining.HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. [23180].
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